Figure 2 | Scientific Reports

Figure 2

From: Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells

Figure 2

The effect of knockdown of COX-1 and COX-2 on Mbnl1 mRNA and MBNL1 protein expression in C2C12 cells. Western blotting (a,b,g,h) and real-time RT-PCR analysis (c–f) were performed using undifferentiated C2C12 cells or C2C12 cells differentiated for 5 days. The cells were treated with siRNA against COX-1 (siCOX1-a or siCOX1-b), COX-2 (siCOX2-a or siCOX2-b), both COX-1 and COX-2 (siCOX1a + 2a or siCOX1b + 2b), or control siRNA (siCont). Undifferentiated C2C12 cells were examined 2 days (mRNA levels) or 3 days (protein levels) after the transfection. Differentiated C2C12 cells were examined on day 5 of differentiation. Expression levels of COX-1, COX-2, Mbnl1 mRNA and MBNL1 protein are normalized to those of Gapdh and GAPDH, respectively, and also to siCont-treated cells. Mean and SD (n = 3 and 6 culture dishes for real-time RT-PCR and Western blotting, respectively) are indicated with individual values in red dots. *p < 0.05, **p < 0.01 and ***p < 0.001 by Student’s t-test with Bonferroni multiple comparison correction.

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