Figure 6 | Scientific Reports

Figure 6

From: Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells

Figure 6

Knockdown of COX-1 suppresses methylation of CpG dinucleotides in MeR2 enhancer region. (a) Methylation patterns of the MeR2. C2C12 cells were transfected with the siRNA against COX-1 (siCOX1-a) or control siRNA (siCont), and DNA was extracted on differentiation day 3. Then the samples were treated with bisulfite sodium and cloned into the TA cloning vector for sequencing. For methylation analysis, 14 independent clones were sequenced for each group, and methylations of 14 CpG dinucleotides in MeR2 were analyzed. Methylated and unmethylated CpG sites are shown in closed and open circles, respectively. (b) The number of demethylated CpG in MeR2 was counted individually at positions 1 to 14, and is plotted. The mean and SEM are indicated. ***p < 0.001 by paired Student’s t-test.

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