Figure 7 | Scientific Reports

Figure 7

From: Inhibition of cyclooxygenase-1 by nonsteroidal anti-inflammatory drugs demethylates MeR2 enhancer and promotes Mbnl1 transcription in myogenic cells

Figure 7

The effects of knockdown of COX-1 or COX-2 on Tet1, Tet2, Tet3 and Dnmt3a mRNA expression in C2C12 cells. Real-time RT-PCR analysis was performed using undifferentiated C2C12 cells (a,b) or C2C12 cells differentiated for 5 days (c,d). The cells were treated with siRNA against COX-1 (siCOX1-a or siCOX1-b), COX-2 (siCOX2-a or siCOX2-b), both COX-1 and COX-2 (siCOX1a + 2a or siCOX1b + 2b), or control siRNA (siCont). Undifferentiated C2C12 cells were examined on 2 days after the transfection. Differentiated C2C12 cells were examined on differentiation day 5. Expression levels of Tet1, Tet2, Tet3, and Dnmt3a mRNA are normalized to that of Gapdh and also to siCont-treated cells. Mean and SD (n = 3 culture dishes) are indicated with individual values in red dots. *p < 0.05, **p < 0.01 and ***p < 0.001 compared to siCont by Student’s t-test with Bonferroni multiple comparison correction.

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