Figure 7
From: Two new, near-infrared, fluorescent probes as potential tools for imaging bone repair
NanoAu-Pam selectively binds to osteoblasts. The specificity and selectivity of nanoAu-Pam were evaluated by incubating the probe with co-cultured human prostate adenocarcinoma cells (LNCaP) and osteoblast cells (7F2). (A) A miniature fluorescence of hydroxyapatite (HA) was observed after 7 days in culture. (B) After 28 d of culture, nanoAu-Pam exhibited intense fluorescence of HA deposits on 7F2 cells presumed to have undergone osteogenesis. Blue arrows denote the NIR fluorescent probe binds to HA accumulation. The distribution of fluorescence is consistent with the osteoblast cell location. Bars in (A,B) are 100 µm.
