Figure 4
Analysis of treatment effects by immunoblotting. (A) Effects of arsenite (As), antimonite (Sb) and OSM on signaling pathways. SIK cultures were treated at confluence for 7 days with 3 µM arsenite, 6 µM antimonite, 50 ng/ml OSM or combinations as indicated, then harvested for immunoblotting with the indicated antibodies. (B) Alteration of ephrin receptor levels by arsenite (As), antimonite (Sb) and OSM. SIK cultures were treated at confluence for 6 days with 3 µM arsenite, 6 µM antimonite or 50 ng/ml OSM, then harvested for immunoblotting. For panel A, the same set of treated cell extracts was run on parallel gels, blotted and probed with the indicated antibodies. For panel B, a different set of cell extracts was run on parallel gels, blotted and blotted probed for the ephrins. Original images of the blots and molecular weight markers are shown in Supplementary Figure 4 from which the relevant areas of interest were cropped (separated by horizontal lines above).
