Figure 4 | Scientific Reports

Figure 4

From: PDCD4 controls the G1/S-phase transition in a telomerase-immortalized epithelial cell line and affects the expression level and translation of multiple mRNAs

Figure 4

Pdcd4 knockdown induces transcriptome-wide changes of mRNA expression. (a) Heat map of all mRNAs whose expression levels were significantly altered (padj value < 0.05) after silencing of PDCD4 by a log2-fold change > 0.5 or <−0.5. The individual columns represent the results of three independent samples from cells transfected with control siRNA (control) and two independent samples of cells transfected with PDCD4 siRNA-2 (si-2). Selected genes are marked on the right side. (b) Real-time PCR analysis of selected up- and down-regulated RNAs. The columns indicate mRNA abundance in control siRNA (black bars) and PDCD4 siRNA-2 (grey bars) treated RPE cells. Individual expression levels determined from three independent biological replicates are marked by white dots. Asterisks indicate statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; students-t test).

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