Table 3 Primers used for Real-Time qPCR expression analysis. Forward and reverse sequences are given, along with product size in base pairs (bp) and annealing temperature (Tm°C).
Gene Name | Forward | Reverse | Product Size (bp) | Tm (°C) |
|---|---|---|---|---|
Down-Regulated | ||||
CYP1A | GACAGGCCAGTGGAGATGG | TTTGTGCAGAGGATCGTGG | 200 | 58 |
M1IP1 | CTGATGAGGTGTGGGACTGC | GGCCTTGTGGTCATCTTGGA | 128 | 60 |
PSMB2 | ATGGCCCCGGTCTCTACTAC | ACACGCATGAGGCATTTCTCT | 146 | 60 |
NLRC3 | AAATGGTCTGACTGGTCGCT | CATCAGAGCAGAACAGACTCCA | 197 | 59 |
Up-Regulated | ||||
HMGCS1 | GATTGGAGATCAGCAGGGAGG | CCAAGGTGGCAAAAGTACAACTC | 182 | 60 |
CYP51A1 | TGGGGCGTGTGTTTTGAGAG | CACCACGGATGTCAGGTTGT | 129 | 55 |
EBP | CTCCACGGTCCCAACAAGAG | TGGTGTCCGTGCTATCTCTG | 188 | 57 |
SQLE | AGCCCCGTCGTCATAGAGAT | GGCGATGCGTACAACATGAG | 120 | 55 |
BTN2A2 | AGCCTGCTGTTCATCCTCAT | TGATCCAGAAGAAGCCGAGG | 187 | 55 |
Housekeeping | ||||
18S | TACAGTGAAACTGCGAATGG | GCATGGGTTTTGGGTCTG | 153 | 60 |
ACTB | GGCCAACAGGGAAAAGATGA | ACCGGAGTCCATGACGATAC | 130 | 59 |
EF1α | TGACAACGTCGGCTTCAACA | GGGTGGTTCAGGATGATGAC | 135 | 60 |
RNAPOL | GCCATGACACCCAGCTAACA | GCAACGTGTGTCCGTGTTTT | 157 | 60 |
RPS20 | AGCCGCAACGTCAAGTCT | GTCTTGGTGGGCATACGG | 98 | 60 |
