Figure 1

Positive control plasmid for pyrosequencing. The Sanger sequencing peak map (a) and the pyrosequencing peak map in complement strand (b) for the positive control clone that contains mt.5703T and mt.5727G alleles. The Sanger sequencing peak map (c) and the pyrosequencing peak map in complement strand (d) for the positive control clone that contains mt.5703 A and mt.5727 T alleles.