Table 3 Polymerase chain reaction primers used in the present study.

From: Inhibition of Porphyromonas gulae and periodontal disease in dogs by a combination of clindamycin and interferon alpha

Specific primer set

Sequence (5′-3′)

Size (bp)

References

Universal primer

PA

AGA GTT TGA TCC TGG CTC AG

315

61

PD

GTA TTA CCG CGG CTG CTG

Detection of P. gulae

TTG CTT GGT TGC ATG ATC GG

314

24

GCT TAT TCT TAC GGT ACA TTC ACA

Specification of fimA type

Type A fimA

   Pgfim-AF

TGA GAA TAT CAA ATG TGG TGC AGG CTC ACG

190

25

   Pgfim-AR

CTT GCC TGC CTT CAA AAC GAT TGC TTT TGG

Type B fimA

   Pgfim-BF

TAA GAT TGA AGT GAA GAT GAG GGA TTC TTA TGT

357

25

   Pgfim-BR

ATT TCC TCA GAA CTC AAA GGA GTA CCA TCA

Type C fimA

   Pgfim-CF

CGA TTA TGA CCT TGT CGG TAA GAG CTT GGA

631

8

   Pgfim-CR

TGT GGC TTC GTT GTC GCA GAA TCC GGC ATG

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