Figure 1
From: Evoked and spontaneous pain assessment during tooth pulp injury
Changes in tooth morphology and trigeminal ganglia transcript levels 1 day following pulp injury. (A) Intact first maxillary molar from a sham animal and (B) injured maxillary molar with pulp exposure evident. Dotted outline marks the molar and the arrow indicates the exposure site. Healthy pulp is still evident on either side of the opening, and compacted foreign material (next to arrow) was present in the cavity. Arrowhead marks an area showing a potential periapical lesion typically seen in tooth infections. (C) Violin plot of quantified fluorescence intensity measured in arbitrary units (a.u.) defined in ImageJ for TrpV1, Tlr4, TrpA1, and Mrgprd in the trigeminal ganglia of injured (blue, n = 3 mice) or sham/naive (red, n = 3 mice) mice. (D–G) Representative images of RNAscope in situ hybridization following sham and (H–K) 1 day after dental pulp injury. **** is p < 0.0001 for an unpaired T-test.
