Figure 3
From: Evoked and spontaneous pain assessment during tooth pulp injury
Facial Von Frey apparatus, mechanosensory response scores, and threshold changes following pulp exposure. (A) The mice were placed in the chamber for testing, which has adjustable openings that can be closed between tests and allows mice to put their head past the bars if they choose. When mice choose to expose their faces they are stimulated as depicted with a von Frey hair. Image created at Biorender.com. (B) After dental pulp injury (blue) there is a significant increase in mechanosensory response scores across all of the filaments tested, which is not observed in sham-treated mice (red). This is evident from day 4 onward on the ipsilateral side, and on day 2 onward on the contralateral side. (C) Raw thresholds are significantly different from normality according to the Shapiro-Wilks Test, but a decrease with time is apparent in the injured group not observed in sham mice. (D) After log transformation of threshold data to conform to normality, we find a significant decrease in threshold following pulp exposure on both sides, evident from day 4 and significantly lower than shams at day 6. *Indicates p < 0.05 for the indicated time point versus baseline (Dunnett’s post-hoc). Square shapes with black borders and lighter data points indicate females. (E) Saline treated mice had a significant decrease in their threshold at both time points following the injury, which did not occur in meloxicam treated mice. *Indicate p < 0.05 by Dunnett’s post-hoc test. All males used in panel E.
