Table 4 Random mutagenesis of the metAP gene of S. thermophilus SMQ301 and resistance to DT1.
Name | Position in the gene | Mutation | Codon change | Amino acid change | EOP | EOP after complementation |
|---|---|---|---|---|---|---|
MetAP_S7 | 169 | C>A | CAG>AAG | Q57K | <×10−6 | 5 × 10−1 |
MetAP_S11 | 503 | C>A | GCG>GAG | A168E | <×10−6 | 3 × 10−3 |
MetAP_S14 | 486 | T>C | TAT>TAC | Y162Y | <×10−6 | 5 × 10−2 |
620 | A>G | GAG>GGA | E207G | |||
623 | A>G | GAG>GGA | E208G | |||
MetAP_S21 | 676 | G>T | GGA>TGA | G226X | <×10−6 | 3 × 10−1 |
MetAP_S32 | 698 | C>A | CCA>CAA | P233Q | <×10−6 | 7 × 10−1 |
MetAP_S36 | 698 | C>T | CCA>CTA | P233L | <×10−6 | 6 × 10−2 |
MetAP_S43 | 37 | A>G | ATG>GTG | M13V | <×10−6 | 2 × 10−1 |
240 | A>G | GCA>GCG | A80A | |||
484 | T>G | TAT>GAT | Y162D | |||
MetAP_S44 | 600 | A>G | GGA>GGG | G200G | <×10−6 | 1.0 |
618 | T>A | CAT>CAA | H206Q | |||
MetAP_S45 | 458 | T>C | CTT>CCT | L153P | <×10−6 | 1.1 |
MetAP_R22 | 683 | T>A | GTC>GAC | V228D | <×10−6 | 1.0 |
