Figure 5
Fibril stability of PrPSc from transgenic mice expressing elk M132 prion protein. (a,b) Stability of PrPSc from first passage mice, normalized to either the 0.25 M data point (a) or the 1 M data point (b). Curves are averaged across 4 (MM, LL groups) or 5 (LM group) mice per group with the curve for each mouse reflecting the mean across technical replicates. (c,d) Stability of PrPSc from second passage mice, normalized to either the 0.25 M data point (c) or the 1 M data point (d). Curves are averaged across 5 (LM-P2a, MM-P2 groups), 6 (LM-P2b), or 8 (LL-P2b) mice, with the curve for each mouse reflecting the mean across technical replicates. [GdnHCl]1/2 = Fraction of PrPSc remaining (0.5). In each case, error bars reflect the SEM (standard error of the mean). (e,f) Comparison of [GdnHCl]1/2 values of PrPSc across first and second passage mice, normalized to either the 0.25 M data point (e) or the 1 M data point (f), as displayed in Table 2. LM values represent those collected from LM-P2b mice. Letters were added to correspond to the assessment of statistical significance described in Table 2.
