Figure 1 | Scientific Reports

Figure 1

From: Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans

Figure 1

Expression of doxycycline-inducible fluorescent proteins in the yeast and filamentous forms of C. albicans. (A) The doxcycline-induced GFPmut2 and mCherry expression in yeast and filamentous forms of C. albicans. C. albicans cells from strains WCL101 and WCL103 were grown to exponential phase and for 3 hours after their transfer to either YPD at 30 °C (yeast) or YPD with 10% FBS at 37 °C (hyphae), both of which contain 40 μg/ml doxycycline. The fluorescent signals from the cells were observed under a fluorescence microscope. The bar indicates 10 μm. (B) Co-expression of GFPmut2 and mCherry induced by doxycycline in yeast and filamentous forms of C. albicans. GFPmut2 and mCherry were co-expressed by doxycycline in C. albicans cells from strain WCL105. The cells were grown in the conditions as in those used in (A). The fluorescent signals from this strain were detected by fluorescent microscopy. The bar indicates 10 μm.

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