Figure 3 | Scientific Reports

Figure 3

From: Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans

Figure 3

Expression of the doxycycline-induced CaCdc11-GFPmut2 and mCherry-CaCdc10 fusion proteins localized at the septum of C. albicans. (A) Examination of the expression of CaCdc11-GFPmut2 and mCherry-CaCdc10 in the presence of doxycycline by western blotting. C. albicans cells from strains WCL114, WCL115, and WCL116 were grown in YPD with (+) or without (−) 40 µg/ml doxycycline for 3 hours. The expression of GFPmut2 or/and mCherry in strains WCL101, WCL103, and WCL105 was used as a control. (B) CaCdc11-GFPmut2 and mCherry-CaCdc10 were localized at the septum of strains WCL115 and WCL116, respectively. Co-localisation of mCherry-CaCdc10 and CaCdc11-GFPmut2 was visualized at the septum of the yeast form under a fluorescence microscope. White arrows indicate areas of fluorescent fusion at the septum. Blue arrows indicate the part of fluorescent fusion in vacuoles. The bar indicates 10 μm.

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