Table 1 C. albicans strains used in this study.

From: Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans

Strain

Parent

Genotype

Plasmidsa

Protein expresseda

Reference

SC5314

Wild-type strain

(Gillum et al.24)

WCL101

SC5314

ADH1/adh1::PTET-GFPmut2:SAT1

pNIM1-NC

GFPmut2

This study

WCL102

SC5314

TDH3/tdh3::PTET-GFPmut2:HygB

pWTN1

GFPmut2

This study

WCL103

SC5314

TDH3/tdh3::PTET-mCherry:HygB

pWTN2

mCherry

This study

WCL104

WCL101

ADH1/adh1::PTET-GFPmut2:SAT1 TDH3/tdh3::PTET-GFPmut2:HygB

pWTN1 & pNIM1-NC

GFPmut2

This study

WCL105

WCL102

ADH1/adh1::PTET-GFPmut2:SAT1 TDH3/tdh3::PTET-mCherry:HygB

pWTN2 & pNIM1-NC

mCherry & GFPmut2

This study

WCL106

SC5314

TDH3/tdh3::PTET -CDC42-mCherry (1-159):HygB

pWTN1-6H-CDC42-RN

Cdc42-RN

This study

WCL107

SC5314

ADH1/adh1::PTET -mCherry (160-237) -RDI1:SAT1

pNIM1-RC-RDI1

RC-Rdi1

This study

WCL108

WCL106

TDH3/tdh3::PTET -CDC42-mCherry (1-159):HygB ADH1/adh1::PTET -mCherry (160-237) -RDI1:SAT1

pWTN1-6H-CDC42-RN & pNIM1-RC-RDI1

Cdc42-RN & RC-Rdi1

This study

WCL109

SC5314

TDH3/tdh3::PTET -mCherry (1-159):HygB

pWTN1-RN

RN

This study

WCL110

SC5314

ADH1/adh1::PTET -mCherry (160-237):SAT1

pNIM1-RC

RC

This study

WCL111

WCL109

TDH3/tdh3::PTET -mCherry (1-159):HygB ADH1/adh1::PTET -mCherry (160-237):SAT1

pWTN1-RN & pNIM1-RC

RN & RC

This study

WCL112

WCL109

TDH3/tdh3::PTET -mCherry (1-159):HygB ADH1/adh1::PTET -mCherry (160-237) -RDI1:SAT1

pWTN1-RN & pNIM1-RC-RDI1

RN & RC-Rdi1

This study

WCL113

WCL106

TDH3/tdh3::PTET -CDC42-mCheery (1-159):HygB ADH1/adh1::PTET -mCherry (160-237):SAT1

pWTN1-6H-CDC42-RN & pNIM1-RC

Cdc42-RN & RC

This study

WCL114

SC5314

TDH3/tdh3::PTET-mCherry-CDC10:HygB

pWTN2-mCherry-CDC10

mCherry-Cdc10

This study

WCL115

SC5314

ADH1/adh1::PTET-CDC11-GFPmut2:SAT1

pNIM1-CDC11-GFPmut2

Cdc11-GFPmut2

This study

WCL116

WCL114

TDH3/tdh3::PTET-mCherry-CDC10:HygB ADH1/adh1::PTET-CDC11-GFPmut2:SAT1

pWTN2-mCherry-CDC10 & pNIM1-CDC11-GFPmut2

mCherry-Cdc10 & Cdc11-GFPmut2

This study

WCL117

SC5314

TDH3/tdh3::PTET -mCherry (1-159)-CDC42:HygB

pWTN1-RN-CDC42

RN-Cdc42

This study

WCL118

WCL117

TDH3/tdh3::PTET -mCherry (1-159)-CDC42:HygB ADH1/adh1::PTET -mCherry (160-237) -RDI1:SAT1

pWTN1-RN-CDC42 & pNIM1-RC-RDI1

RN-Cdc42 & RC-Rdi1

This study

WCL125

WCL117

TDH3/tdh3::PTET -mCherry (1-159)-CDC42:HygB ADH1/adh1::PTET -mCherry (160-237):SAT1

pWTN1-RN-CDC42 & pNIM1-RC

RN-Cdc42 & RC

This study

  1. aRN indicated as mCherry (1–159) and RC indicated as mCherry (160–237).
Back to article page