Figure 1
From: A simple and effective method to discern the true commercial Chinese cordyceps from counterfeits
The sequence alignments used to design the primer pairs for Chinese cordyceps (DongChongXiaCao) detection. All the sequences were subjected to a BLAST search using DNAMAN software, and the black boxes indicate identical nucleotides. The nucleotide sequences of primers employed in this duplex PCR system are listed below each alignment. (A) Alignment of internal transcribed spacer sequences from Ophiocordyceps sinensis and its related species. In total, 78 ITS nucleotide sequences of O. sinensis and other related species taken from GenBank were aligned and compared. The positions of the CITS-F10′ and CITS -R10′-2 primers are indicated by bold black boxes. (B) Alignment of COI gene sequences from its host Hepialiae species. In total, eight nucleotide sequences of the COI gene were aligned and compared. The positions of the COI-F and COI -R primers are indicated by bold black boxes.
