Figure 2
From: MultiFRAGing: Rapid and Simultaneous Genotyping of Multiple Alleles in a Single Reaction
Fragment Analysis PCR plots from wild-type samples and a pool of samples amplified together. (A) Wild-type controls for size comparison with two targets separated by different dyes. (B) Two targets are separated based on different dyes, showing a 2 bp or 1 bp deletion when compared to wild-type sizes. (C) Wild-type controls for size comparison with three targets separated by different dyes. (D) Three targets are separated by dye color, and show a 2 bp deletion, 4 bp insertion, and 1 bp deletion compared to wild-type sizes.
