Figure 3
From: Color-specific porosity in double pigmented natural 3d-nanoarchitectures of blue crab shell

Mapping the Raman intensity distribution of the astaxanthin (ATX) v1(C=C) band at 1514 cm−1 and its non-covalently bound counterpart (ncb-ATX) at 1492 cm−1 from spectra collected from the blue Callinectes sapidus and green Carcinus aestuarii crab cuticle surface. Excitation: 532 nm. A1,2 and B1,2 show the light microscopy images taken via Raman microscope with 5x or 20x objective respectively, while A3 and B3 display the two pigments interplay over the mapped area highlighted in rectangle in A2 and B2. A4 and B4 show the rough Raman signal of the respective cuticle at the cross-hair of the maps. Note the spatial interplay of the two pigments contribution.