Figure 4
From: Color-specific porosity in double pigmented natural 3d-nanoarchitectures of blue crab shell

Pigments distribution in the cross section of blue cuticle (a) along with the normal-to-surface direction: (b,c) micrographs taken via Raman microscope during measurements; (d) RR spectra of ATX collected from surface points along the shell transect, from margin to the inner layers (shell depths) from 20 to 420 µm as indicated on each spectrum; (e) ATX Raman C=C mode (1514 cm−1) intensity distribution against depth; the data fit showed a Lorentzian profile with two components, peaking at 130 and 260 μm respectively; (f) RR spectra of ncb-ATX taken from cross section surface (shell depth from 20 to 240 μm); and its main band (1492 cm−1) intensity variation along cross section (g), showing the highest intensity in epicuticle. Error bars show the standard deviation.