Figure 3
Histopathological changes in the cardiovascular system in the three groups. Hematoxylin-eosin staining by light microscopy showed that cardiac muscle fibers were integrated and arranged in an orderly manner in the control group (A,B). In Group OSAHS, massive collapsed and fissured cardiac muscle fibers were detected. (A,B) The arrangement of cardiac muscle fibers was somewhat disordered, and few muscle fibers were collapsed and fissured in Group MAD. (A,B) The ultrastructure of the capillary among the myocardial fibers in transmission electron microscopy showed the significant thickness of the basement membrane (* shown in C,D), the pyknosis of nuclear chromatin and the margination phenomenon under the nuclear membrane (arrow shown in C,D) in Group OSAHS; The pyknosis of nuclear chromatin and the margination phenomenon under the nuclear membrane and the slight thickness of the basement membrane in Group MAD. (C,D) The ultrastructure of myocardial fibers showed that the myofibrils were arranged in an orderly manner in the control group. Dissolved and destroyed myofibrils were in disorder in Group OSAHS (arrow shown in E), and the fusion and disappearance of the cristae along with the membrane of mitochondria are shown (hollow arrow shown in E). These results suggest that myocardial injury associated with OSAHS can be rescued by MADs.
