Figure 2

Immunohistochemistry for PTH-expressing cells in adipose tissues surrounding the parathyroid glands of secondary hyperparathyroidism patients. Adipose tissues surrounding the parathyroid glands of secondary hyperparathyroidism patients were stained with anti-PTH or anti-GCM2 antibody. Nuclei were stained with hematoxylin. Representative specimens are shown. Filled arrows indicate colonies of PTH- or GCM2-expressing cells. Empty arrows indicate adipocytes. (a) stained with anti-PTH antibody. A low-magnification view. Bar, 200 μm. (b) an enlarged view of a part of (a). Bar, 50 μm. (c) stained with anti-GCM2 antibody. A low-magnification view. Bar, 200 μm. (d) a negative control stained without primary antibodies.