Figure 2
Effects of prelimbic (PL) cortex PKC inhibition by chelerythrine (Che) on the contextual fear memory persistence without prior reactivation. (A) The general experimental design used. Animals were initially familiarized to Context A. A day later, the context was paired with three shocks (US). On the next day, 6 h after omitting memory reactivation (neutral and unpaired Context B exposure), the animals received a bilateral infusion of vehicle (Veh) or Che (3.0 nmol) intra-PL cortex. One and seven days later, the animals were re-exposed to the paired context (Tests A1 and A2) to assess the Che effects on memory. (B) Che effects on memory persistence when given 6 h after omitting memory reactivation. Che-treated animals presented freezing time similar to controls during Test A2, suggesting no changes in memory persistence. Values are expressed as mean ± S.E.M (6/group). The fence (hashtag) denotes a statistically significant difference (P < 0.05) from Tests A1 and A2 relative to the no reactivation session in both groups (mixed ANOVA followed by the Tukey test).
