Figure 2 | Scientific Reports

Figure 2

From: Gender-specific changes in energy metabolism and protein degradation as major pathways affected in livers of mice treated with ibuprofen

Figure 2

Effects of ibuprofen treatment on 20S proteasome and immuno-proteasome expression levels and activities in mice liver. (A) Changes in level of 26S proteasome subunits (β1, β2 and β5) and immunoproteasome subunits (β1i and β5i) obtained by mass spectrometry. Western blots of proteasome levels (PSMA6, β1i, β2i and β5i) and total protein (used as loading controls) in (B) Male liver, and (C) Female liver. Value are mean ± SE; n = 4–6 per group. *p < 0.05. (D) 26S proteasome (β1, β2 and β5) activities of liver lysates from male mice. (E) 26S proteasome (β1, β2 and β5) activities of liver lysates from female mice. (F,G) β1i and β5i immune-proteasome activities of male and female liver lysates. Value are mean ± SE; n = 6 per group. *p < 0.05.

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