Figure 3
Translation of MaEST EW753059.1 aligned with sequences determined by tandem mass spectrometry sequencing of tryptic fragments from purified MaAFP. The 87-residue open reading frame from the EST (with the signal sequence removed) is shown sequentially in bold as four tryptic fragments of masses 3442.42, 1688.74, 460.24 and 1211.57. Gly residues predicted to form the core are highlighted orange. Above it are the tandem MS/MS sequences that exactly correspond to and support the EST sequence. Below it are homologous fragments producing some of the higher total ion counts (excluding trypsin autolysis fragments) numbered #1 to #7 shown in Supplementary Fig. S1. Differences in these sequenced fragments relative to the bolded EST translation are highlighted yellow. Residues that were inferred from b2 ion masses are in lowercase, and undetermined regions of unknown length are represented by question marks. The predicted (EST) and observed (MS/MS fragment) monoisotopic H+1 masses (in Da) are indicated on the right.
