Figure 7
Summary of bioinformatic analysis of miRNA transfections and subsequent differential protein expression in XXL-USSC. (A) Bioinformatic workflow. Proteins regulated in 3d XXL-USSC upon transfection with mimics/inhibitors (left Venn diagram) were filtered for predicted targets (red arrow, using all 12 algorithms from miRWalk 2.0) and cross-analysed with inversely regulated predicted targets in non-transfected XXL-USSC (right Venn diagram). (B) For each miRNA mimic/inhibitor transfection, numbers of quantified proteins in transfected 3d XXL-USSC, predicted target proteins hereof, proteins with altered abundance (high and low) upon transfection, predicted targets hereof, and overlap (light green cell/column) with inversely regulated predicted targets (changes in abundance at 3d and/or 7d XXL incubation) in XXL-USSC are given.
