Table 2 The primers and probes used for qPCR.
Gene Name | Primer and Probe Sequence (5′ to 3′) | Amplicon Size (bp) | Annealing Temperature (°C) |
|---|---|---|---|
D-loop | F: 5′ACCCCTGCCTGTAATGTACTTC3′ R: 5′CACGGACTAAAGAGGGGAAGAT3′ Probe: 5′TTCTTTCCCCCTACACCCCTCGCCCT3′ | 183 | 60 |
ATP6 | F: 5′ATTCTCAAGCCCCTGCCTAC3′ R: 5′TCAGAGTTGGATGGTGGAGAGG3′ Probe: 5′CCTCCCATCACTCCTTCTTCCAGCCCTC3′ | 124 | 60 |
ND6 | F: 5′TAACAACAAACCTCACCCAGCC3′ R: 5′GTGTGTCTTTTGCTCGGTTGGA3′ Probe: 5′AGCCACCAAAAACAACCCAACCCCAC3′ | 95 | 60 |
GCG | F: 5′GTGGAGGGCTGATAAAACACAAT3′ R: 5′TCCAACTCCTTGACCTCTATCC3′ Probe: 5′TTCAGCCCTCAGCATTCAGTCCCATT3′ | 205 | 60 |
