Figure 2 | Scientific Reports

Figure 2

From: Whole embryo culture, transcriptomics and RNA interference identify TBX1 and FGF11 as novel regulators of limb development in the mouse

Figure 2

Gene expression dynamics in forelimb buds developing ex utero. (A) Overview of approach and computational analysis. We used the forelimb bud of E10.5 embryos at time 0 as a reference and attributed the value 0 to all the genes expressed in this sample of reference (Reference, Histogram baseline). The relative expression level of genes differentially expressed during the ex utero development of the FB of E10.5 mouse embryos after 1 h, 3 h and 6 h of culture, is normalized to reference genes expressed in FBs of freshly dissected E10.5 embryos (histogram baseline). (B) Expression profile of genes differentially regulated during the ex utero forelimb bud development of E10.5 mouse embryos. After 1 and 3 hours of culture we identified 616 genes with a linear dynamic expression through the time course of ex utero development. However, most of these genes saw their expression going back to its original level after 6 hours of culture. The Y axis represents a normalized expression variation.

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