Figure 3

Impact of embryo culture on tissue growth. (A) Percentage of Annexin-V positive cells in the forelimb bud of E10.5 mouse embryos before ex utero culture and after 1, 3 and 6 h of culture. Results of the FACS analysis are represented in the right panel as histogram (%± SEM). In the left panel, a representative FACS dot plot of Annexin-V + cells at each time point for one mouse embryo. (B) Overview of approach and computational analysis. The functional analyses were generated through the use of IPA (QIAGEN Inc., https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis). During the first hours of mouse embryo culture in a controlled system, 39 out of 83 genes display a similar expression dynamic and are associated with cell death and survival processes. (C) Percentage of H3 phosS10 positive cells in the forelimb bud of E10.5 mouse embryos before ex utero culture and after 1, 3 and 6 h of culture. Results of the FACS analysis are represented in the right panel as histogram (%± SEM). In the left panel, a representative FACS dot plot of H3 phosS10⁺ cells at each time point for one mouse embryo.