Figure 4

Genes differentially expressed in ex utero and in utero developing forelimb buds. (A) Overview of approach and computational analysis. Expression profiles of genes differentially regulated during the ex utero and in utero forelimb bud development of E10.5 and E12.5 mouse embryos. The forelimb bud of E10.5 embryos at time 0 is used as a reference and the value 0 was attributed to all the genes expressed in this sample of reference. After 1 and 3 hours of culture of E10.5 and E12.5 embryos and in the forelimb of non-cultured E12.5 embryos we identified 675 probe sets with a linear dynamic expression through the time course of ex utero and in utero forelimb development. The Y axis represents a normalized expression variation. (B) Genes differentially expressed in the forelimb of in utero and ex utero developing embryos were associated with mainly 9 distinct different canonical pathways identified by IPA (P < 0.05). The y-axis represents the −log of P-values calculated by Fischer exact test.