Figure 7

Investigation of Fgf11 and Tbx1 implication in forelimb development. (A) Relative expression profiles of Fgf11 and Tbx1 in the forelimb of E10.5 (left) and E12.5 (right) mouse embryos after 1 and 3 hours of culture. RT-qPCR on separated cells using Rps9 as a reference gene. (B-C) Relative expression profiles of Fgf11 and Tbx1 in the forelimb of E10.5 mouse embryos that have been transfected with either the siRNA control (siRNA CTL) or the siRNA against Ffg11 or Tbx1 (siFgf11 or siTbx1, respectively) at the embryonic stage E9.5 and cultured 24 h ex utero. (D) E10.5 mouse embryos co-injected with the siCTL tagged with CY3 and siRNA targeting either Fgf11 or Tbx1 or the two simultaneously in the left forelimb of E9.5 embryos cultured for 24 h in the roller culture system were imaged using a Zeiss Discovery V8 fluorescence stereomicroscope. (E) Measurements of the surface of the left forelimb of E9.5 mouse embryos cultured 24 h after siRNA administration normalized by the surface of the untreated right forelimb of mouse embryos. (F-G) Relative expression levels of Sox6 and Sox9 in the FBs of ex utero developing embryos 24 h after the transfection with siRNA against Fgf11 or Tbx1. (H-I) Relative expression levels of Ffg11 (H) and Tbx1 (I) in the forelimb of E10.5 mouse embryos that have been transfected with either the siRNA control (siRNA CTL) or the siRNA against Sox6 at the embryonic stage E9.5 and cultured 24 h ex utero. Each graph represents the mean value of at least 3 independent experiments ± SEM. *P-values <0.05 (*) or P < 0.001 (***).