Table 1 Plasmids used in this study.

From: Identification of a novel trigger complex that facilitates ribosome-associated quality control in mammalian cells

Name

Features

Sourse

pcDNA3.1(+)-V5-GFP-FLAG-HIS3

V5-GFP-FLAG-HIS3 reporter

Matsuo and Ikeuchi et al., 2018

pcDNA3.1(+)-V5-GFP-K(AAA)24-FLAG-HIS3

V5-GFP-K(AAA)24-FLAG-HIS3 reporter

Matsuo and Ikeuchi et al., 2018

pcDNA3.1(+)-3xFLAG-ASCC3

ASCC3 transcript variant 1 expression vector (NM_006828.4)

This study

pcDNA3.1(+)-HA-ASCC3

ASCC3 transcript variant 1 expression vector (NM_006828.4)

This study

pcDNA3.1(+)-3xFLAG-ASCC2

ASCC2 transcript variant 4 expression vector (NM_001369921.1)

This study

pcDNA3.1(+)-HA-ASCC2

ASCC2 transcript variant 4 expression vector (NM_001369921.1)

This study

pcDNA3.1(+)-3xFLAG-ASCC1

ASCC1 transcript variant 2 expression vector (NM_001198800.3)

This study

pcDNA3.1(+)-HA-ASCC1

ASCC1 transcript variant 2 expression vector (NM_001198800.3)

This study

pcDNA3.1(+)-3xFLAG-TRIP4

TRIP4 transcript variant 2 expression vector (NM_001321924.2)

This study

pcDNA3.1(+)-HA-TRIP4

TRIP4 transcript variant 2 expression vector (NM_001321924.2)

This study

pFUGW-FLAG-ASCC3 shR

shRNA resistant ASCC3 expression vector

This study

pFUGW-FLAG-ASCC3 shR K505R

shRNA resistant ASCC3 K505R expression vector

This study

pcDNA3.1(+)-HA-ASCC2 shR

shRNA resistant ASCC2 expression vector

This study

pcDNA3.1(+)-HA-ASCC2 shR Ub-m

shRNA resistant ASCC2 Ub-m expression vector

This study

pGEX4T2-GST

recombinant protein of GST

This study

pGEX4T2-GST-Ubiquitin

recombinant protein of GST-Ubiquitin

This study

pGEX6P2-GST-prescission site-HA-ASCC2

recombinant protein of HA-ASCC2

This study

pGEX6P2-GST-prescission site-HA-ASCC2-Ub-m

recombinant protein of HA-ASCC2-Ub-m

This study

pcDNA3.1(+)-HA-ZNF598(1-904) shR

shRNA resistant ZNF598 expression vector (NM_178167.4)

This study

pcDNA3.1(+)-HA-ZNF598(ΔRING) shR

shRNA resistant ZNF598 ΔRING expression vector

This study

pcDNA3.1(+)-HA-ZNF598(C29SC32S) shR

shRNA resistant ZNF598 C29SC32S expression vector

This study

pcDNA3.1(+)-HA-ZNF598(1-634) shR

shRNA resistant ZNF598 1-634 expression vector

This study

pcDNA3.1(+)-HA-ZNF598(1-278) shR

shRNA resistant ZNF598 1-278 expression vector

This study

pcDNA3.1(+)-HA-ZNF598(1-246) shR

shRNA resistant ZNF598 1-246 expression vector

This study

pcDNA3.1(+)-HA-ZNF598(1-186) shR

shRNA resistant ZNF598 1-186 expression vector

This study

pcDNA3.1(+)-HA-ZNF598(21-904) shR

shRNA resistant ZNF598 21-904 expression vector

This study

pcDNA3.1(+)-HA-ZNF598(21-278) shR

shRNA resistant ZNF598 21-278 expression vector

This study

pcDNA3.1(+)-Rluc-K24-Fluc

Rluc-K24-Fluc reporter

This study

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