Figure 5 | Scientific Reports

Figure 5

From: Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail

Figure 5

Chemical shift changes as a function of fatty acid chain length. Comparison of chemical shift differences in the 1H-15N HSQC spectra recorded at 600 MHz for (a) apo compared to octanoic acid. (b) Apo versus C10. (b) C10 acid vs C15 acid and (d) C15 vs C19 acids. Significant chemical shift perturbations are color coded as in Fig. 1. In panel (a), the vertical scale has been expanded by four compared to the other panels. For C10–C14 conformational averaging in the C-terminal tail limited the assignments for a number of residues in this region (shaded in grey in b and c). In all cases the protein was at ~100 μM and the fatty acid was present at a concentration of 200 μM in sodium phosphate at pH 6.5.

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