Figure 3

Expression pattern of FAM19A1 in the adult mouse brain. (A) X-gal precipitates (red) were detected in the subset of cortical L2-3 CUX1-positive neurons (green) in FAM19A1 LacZ KI mice. (B) β-galactosidase (green) were identified in the subset of cortical L5 CTIP2-positive neurons (magenta) in FAM19A1 LacZ KI mice. Arrowheads indicate X-gal or β-galactosidase expressing cortical marker cells. (C–K) X-gal staining in the coronal brain sections of a FAM19A1 LacZ KI (+/−) mouse. AO, anterior olfactory nucleus; Apir, amygdalopiriform transition area; BLA, basomedial amygdaloid nucleus; BLP, basolateral amygdaloid nucleus, posterior part; CEn, entorhinal cortex; CPf, piriform cortex; D3V, dorsal 3rd ventricle; FrA, frontal association cortex; L2-3, cortical layer 2-3; L5, cortical layer 5; CA1, 2 and 3, field of CA1, CA2 and CA3 regions of the hippocampus; LaDL, lateral amygdaloid nucleus; LHb, lateral habenular nucleus; LO, lateral orbital cortex; LS, lateral septal nucleus; LV, lateral ventricle; MGN, medial geniculate nucleus; MO, medial orbital cortex; Op, optic nerve layer of the superior colliculus; PMCo, posteromedial cortical amygdaloid nucleus; PrL, prelimbic cortex; Py, pyramidal cell layer of the hippocampus; RG, retrosplenial granular cortex; VO, ventral orbital cortex. Scale bars represent 100 μm in panel A and 500 μm in panels (C–K).