Figure 2
From: Bacterial chemotaxis towards polysaccharide pectin by pectin-binding protein
Expression of chemotaxis towards pectin by gene complementation in strain A1-M5 cells. (A) Chemotaxis of strain A1-M5 cells complemented by genomic fragments from the wild-type strain A1 cells. (B). Chemotaxis of the isolated genomic fragment-complemented strain A1-M5 cells towards pectin. (C) Chemotaxis of strain A1-M5 cells transformed with pKS13-pectin towards pectin. (D–F) Chemotaxis assay of specific gene-complemented strain A1-M5 cells. Chemotaxis towards pectin was observed in sph1117-sph1118-complemented strain A1-M5 cells (D) and sph1119-complemented strain A1-M5 cells (E) but not in the sph2733-complemented strain A1-M5 cells (F). Except for the plate (F), the grey area in the middle of the plate stems from accumulating cells. Each plate assay was replicated three times independently. The profile of each plate is not an image cropped from different parts of the same plate or from different plates.
