Figure 1
From: Recapitulating T cell infiltration in 3D psoriatic skin models for patient-specific drug testing
Inducing the infiltration of CD4+ T cells into HSCs. (a) Method for generation of immunocompetent HSC. (b) 3D-reconstructed whole-mount image of HSCs showing 3D conformation of K14-positive epidermis and CD3-positive T cells with and without the epidermis (DAPI: blue). (c) Quantification of the number and penetration depth of infiltrated T cells in HSCs (µm). (d) Orthogonal segment of T cell-bearing HSCs with the centerline of their initial position on the gel surface as a reference (white dotted line) showing CD3-positive (green) T cells (DAPI: blue). (e) Quantification of the total number of cells that migrated upward (dermis) or downward (acellular gel).
