Figure 2
From: Recapitulating T cell infiltration in 3D psoriatic skin models for patient-specific drug testing
The effect of the epidermis on CD4+ T cell activation. Immunofluorescent staining and flow cytometry analysis of T cell activation markers, CD69 (a) and CD25 (b), in CD3+ T cells isolated from HSCs with the epidermis or without the epidermis (b). (c) Histograms for CD69 (upper panel) and CD25 (lower panel) on CD3+ T cells from HSCs with the epidermis (red) or HSCs without the epidermis (blue). (d) Cytokine secretion from T cell-bearing HSCs with or without the epidermis using cytokine ELISA plate arrays (t-test; *p < 0.01, N = 3).
