Figure 6
From: Recapitulating T cell infiltration in 3D psoriatic skin models for patient-specific drug testing
Epidermal phenotype and cytokine profile of HSCs containing patient-derived T cells. (a) Flow cytometry analysis for intracellular IFNa and IL-17a from patient-derived CCR6+CLA+ skin homing T cells and CCR6−CLA- T cells. (b) Immunofluorescence staining of K16 (red), hBD2 (green), and PPP6c (green) in CCR6−CLA- T cell-bearing HSCs (left panel), CCR6+CLA+ T cell-bearing HSCs (middle panel), and CCR6+CLA+ T cell-bearing HSCs treated with anti-IL17a antibody (right panel). (c) Cytokine secretion measured by ELISA over two days in CCR6−CLA- T cell-bearing HSCs, and CCR6+CLA+ T cell-bearing HSCs. (d) Relative cytokine secretion over two days in CCR6+CLA+ T cell-bearing HSCs, and CCR6+CLA+ T cell-bearing HSCs treated with anti-IL17a antibody.
