Figure 3

Systemic estrogen synthesis inhibition suppresses song production without impacting tutor song copying. (A) Daily number of song bouts before and across the tutoring/treatment period. (B) Birds sing amounts before treatment/tutoring; however, systemic FAD treatment reduces song production (p = 0.012). Circles/orange = saline-treated birds (n = 3); triangles/blue = FAD birds (n = 3). (C) Song similarity is lowest at 49 dph despite treatment (effect of age: p = 0.005; * is relative to 49 dph). (D) At 130 dph, tutor song similarity, accuracy, and sequence similarity, respectively, are all similar across treatments. (E) Change in Wiener entropy at 49 dph (post-tutoring day #5) relative to pre-tutoring values predicts eventual percent song similarity to the tutor at 130 dph, independent of treatment (r2 = 0.903; p = 0.004). *p < 0.05.