Figure 3
From: Fabrication of sharp silicon arrays to wound Caenorhabditis elegans
Efficient wounding of C. elegans with a silicon array. (a) Quantification of the change of reporter gene expression provoked by wounding with a needle (5 left-hand columns), or arrays, worms carrying frIs7 in the wild-type or sta-2 mutant background (circles and triangles, respectively). The fluorescence ratio (Green/TOF in arbitrary but constant units) is shown for the indicated number of worms for each condition (n). The bars indicate the means. (b) Representative images of control (top) and wounded (bottom) adult wild-type worms stained with Hoechst 33258. In the control animals, only staining of the intestinal nuclei is apparent (arrows), while in the array-wounded worms nuclei in the epidermis (arrows) are also stained.
