Figure 4

Cdc5 interacts simultaneously and non-competitively with Spc72^P and Dbf4. Isothermal calorimetry data and analysis for the titration of the Spc72^P peptide (a), its non-phosphorylated version (Spc72) (b), and the Dbf4 peptide (d) into the polo-box domain of Cdc5. (c) Titration of the Spc72^P peptide into the polo-box domain of Cdc5 pre-incubated with the Dbf4 at 1:4 molar excess. (e) Titration of the Dbf4 peptide into the polo-box domain of Cdc5 pre-incubated with the Spc72^P at 1:4 molar excess.