Table 1 The concentrations of cytokines (pg/ml) in the plasma of the 1 stage and 2 stage HLI models.

From: Development of a two-stage limb ischemia model to better simulate human peripheral artery disease

Proteins

1 stage -Sham (N = 6)

1 stage HLI (N = 10)

2 stage -Sham (N = 6)

2 stage HLI (N = 10)

P value

Monocyte Chemoattractant Protein-1 (MCP-1)

ND

39.28 ± 11.77

ND

30.33 ± 20.64

0.697

Interleukin-6 (IL-6)

ND

4.12 ± 1.48

ND

2.36 ± 0.58

0.334

Interleukin-10 (IL-10)

ND

11.81 ± 6.06

ND

8.35 ± 4.27

0.690

Interferon-γ (IFN-γ)

ND

2.62 ± 1.22

ND

0.52 ± 0.34

0.233

Tumor Necrosis Factor (TNF)

ND

5.45 ± 2.93

ND

3.17 ± 1.50

0.591

Interleukin-12p70 (IL-12p70)

ND

21.60 ± 9.62

ND

46.05 ± 25.56

0.825

NO (nitrite + nitrate)

11.20 ± 2.17

61.18 ± 10.50$

21.13 ± 6.07

42.98 ± 20.33

0.031*

  1. The concentrations of cytokines (pg/ml) were measured in the platelet poor plasma (n = 6–10/group) collected at the end of the study using a cytometric bead assay. NO metabolites were assessed by the Griess assay. Data expressed as mean ± standard error of mean (SEM) and analysed by student’s t-test. P values are for two-way comparisons between the 1-stage and 2-stage HLI models. Statistical significance considered as P value ≤0.05. (*)  = ≤0.05, 2-stage HLI compared to 1-stage HLI; ($)  = <0.001, 1-stage HLI compared to shams. Abbreviations: IL, Interleukin; IFN, Interferon; MCP, Monocyte Chemoattractant Protein; NO, total nitrite and nitrate metabolites; TNF; Tumour Necrosis Factor.
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