Figure 3
From: Metabolomics and the pig model reveal aberrant cardiac energy metabolism in metabolic syndrome
CAC metabolites in MS and LD. CAC related intermediate metabolites in the MS and LD heart are identified and quantitatively compared by LC/MS-MS. Data represents 7 LD and 8 MS pig. (a) Content of pyruvate, acetyl CoA, and CAC intermediate metabolites: citrate, aconitate, isocitrate, glyoxylate, a-ketoglutarate (a-KG), succinyl-CoA (SCoA), succinate, ɣ-aminobutyric acid (GABA), glutamate, glutamine, fumarate, malate, oxaloacetate in relative units. The individual metabolites are shown at the top of the diagrams. Values are means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001; LD n = 7, MS n = 8. (b) 2d TLC separation of succinate and malate in LD and MS tissue: the single arrow indicates the position of malate; double arrow indicates succinate. The spot intensity (pixels) of four independent extracts, each from 10 mg of tissue (n = 4 and 4, **p < 0.01) is shown with the bar diagram. Error bars indicate ±SEM. (c) Diagram representing the alterations in the intermediate CAC metabolites in the MS heart together with the CAC connected glutamate-GABA and glyoxylate pathway: acetyl CoA (AcCoA) and the CAC intermediate metabolites citrate (Cit), aconitate (ACC), isocitrate (iCIT), glyoxylate (GLX), a-ketoglutarate (KG), succinyl-CoA (SCoA), succinate (SUC), ɣ-aminobutyric acid (GABA), glutamate (Glu), fumarate (FUM), malate (MAL), and oxaloacetate (OAA). The blue arrows denote diminished metabolites in MS vs. LD; red - high in MS.
