Figure 3 | Scientific Reports

Figure 3

From: Development of Oxytolerant Salmonella typhimurium Using Radiation Mutation Technology (RMT) for Cancer Therapy

Figure 3

Expression and secretion of SspH1:sATF6 fusion protein in KST0650. (a) Schematic map of radiation-inducible SspH1:sATF6 expression and export system (pRS-sATF6). (b) KST0652-expressing SspH1:sATF6 was induced with the indicated dose of γ-radiation, followed by incubation for 2 h at 37 °C. SspH1:sATF6 protein level was determined using western blot analysis with anti-FLAG antibody in the bacterial lysate and bacterial culture supernatant. DnaK was detected and used as the loading control. (c) Expression of SspH1:sATF6 in KST0652 was measured using western blot analysis at the indicated time points after 2 Gy of γ-radiation. Full-length blots are presented in Supplementary information.

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