Table 1 Characterization of 5 duplex PCR assays in Chinese sturgeon.
Group | Locus/GenBank no. | Repeat motif(s) | Primer sequence (5′-3′) | Tm (°C) |
|---|---|---|---|---|
I | CS57/MN401754 | (TTCT)12 | F:GCAACTTATACACAAATACAGTGGGT | 56 |
R:AGCAAGTCCTGTGCCTTATCA | ||||
ZHX43/MN401755 | (TATC)11 | F:TATTGCATGAAGCTGTGCGC | 56 | |
R:AGGGGCGAATGATACTGCAC | ||||
II | ZHX69/MN401756 | (CTTT)10 | F:AGCTGCTTCTACACCGACAC | 56 |
R:GTGCCCAAGATAGCGCAAAA | ||||
AS105/AY921055 | (GATA)9 | F:CGAATGAAATTGACGCAAAC | 56 | |
R:CCATTTATTTTGGCCACCAG | ||||
III | ZHX51/MN401757 | (TATT)11 | F:GCTTTGCGCACTCTTTCCAT | 56 |
R:GGCACTCCACTCCACTGTAC | ||||
AS074/AY921042 | (CTTT)13 | F:AAAGGGAACTTCATCTTTTCCA | 56 | |
R:GTTTTGCCATGCCAATCTTT | ||||
IV | ZHX2/MN401758 | (ATCT)11 | F:TCAGTGCATTAACTTACATTTTGCA | 56 |
R:AGAGTCCTCTTCATGACACACA | ||||
AS078/AY921045 | (CTTT)13 | F:TCTGGATAGCTGGCCTTCTG | 56 | |
R:AACTGTGCAAAAGGGGAAGA | ||||
V | AS026/AY921024 | (GAAA)13 (GACA)11 (GGCA)8 | F:AAAGCGCGCTGTTTGTGT | 56 |
R:AAACAAATCCAGGAGCGAAG | ||||
AS073/AY921041 | (CTAT)12 | F:AGCCCCATCTGCAATACTGT | 56 | |
R:CTGATGGCATATCACATGCTT |
