Figure 3
Effect of taurine on the secondary and tertiary structure of α-casein: Far- UV CD spectra (a), ThT fluorescence intensity (b), near-UV CD spectra (c), intrinsic tryptophan fluorescence (d), acrylamide quenching (e) and ANS fluorescence intensity (f) of α-casein in presence of different concentrations of taurine 0 mM (——), 50 mM (— —), 100 mM (– – –), 250 mM (—•—), 350 mM (—••—), 500 mM (— – –). Acrylamide quenching has been performed only at highest concentration of taurine. Spectra shown are representative of atleast three independent measurements with error rates as mentioned in Table 2.
