Figure 3
ARNO is involved in resistin induced VSMC migration. (a) Secin H3 treatment (15 µM) reduced basal (*p < 0.05, 1-way ANOVA on ranks, n = 11 in triplicates) and prevented resistin dependent VSMC migration into a wound in vitro (*p < 0.05, 1-way ANOVA on ranks, n = 11 in triplicates). (b) Expression of a catalytically inactive ARNO (EK) or an ARNO construct with a non-functional PH-domain (RD) prevented the resistin dependent VSMC migration compared to ARNO wildtype (WT) expressing cells (*p < 0.05, 1-way ANOVA on ranks, n = 5–6 in triplicates). (c) Inhibition of JNK and p38 MAPK impaired resistin (100 ng/ml) induced VSMC migration (*p < 0.05, 1-way ANOVA on ranks, n = 4 in duplicates). Representative photographs of wounded cell areas (scratches) and migrating VSMC (DAPI staining, blue) are shown to the right of all graphs. Bar in photos represents 200 µm. All data are presented as mean + SEM.
