Table 3 Plasmids used in this study.
From: Alternaria alternata uses two siderophore systems for iron acquisition
Plasmid | Content | Reference |
|---|---|---|
pFC332 | tef1(p)::cas9::tef1(t); gpdA(p)::gpdA(t); hph; ampR; AMA1 | Uffe H. Mortensen, Technical University of Denmark |
pFC330 | tef1(p)::cas9::tef1(t); gpdA(p)::gpdA(t); AfpyrG; ampR; AMA1 | Uffe H. Mortensen, Technical University of Denmark |
pFC334 | tef1(p)::cas9::tef1(t); gpdA(p)::sgRNA-AnyA::gpdA(t); Afpyr4; ampR; AMA1 | Uffe H. Mortensen, Technical University of Denmark |
pBV2 | tef1(p)::cas9::tef1(t); gpdA(p):: AapNPS2- sgRNA cassette1::trpC(t); hph; ampR; AMA1 | This study |
pBV3 | tef1(p)::cas9::tef1(t); gpdA(p):: AapNPS2- sgRNA cassette2::trpC(t); hph; ampR; AMA1 | This study |
pBV4 | tef1(p)::cas9::tef1(t); gpdA(p):: AapNPS6- sgRNA cassette1::trpC(t); hph; ampR; AMA1 | This study |
pBV5 | tef1(p)::cas9::tef1(t); gpdA(p):: AapNPS6-sgRNA cassette2::trpC(t); hph; ampR; AMA1 | This study |
Efimov-gpdA(p) | gpdA(p)::trpC(t); ampR; pyr-4 |
