Table 4 Display of the deletion events using the CRISPR/Cas9 strategy.
From: Alternaria alternata uses two siderophore systems for iron acquisition
Strain | WT sequence | Mutant sequence | Mutation |
|---|---|---|---|
SBV2 (Δnps2) | …[273]AGAACTATACATAGTCTTAT[292]… [8594]TTTGCCGTGGACTGTCACCT[8613]… → nps2: 16273 bp, 5372 aa | …[260]TGCCTCAGTCTCC AGAACTATACATAGTCT------------CCTTGGAAGATACGTCAGA CTCA [8633]… | 8321 bp deletion 2757 aa deletion
|
SBV3 (Δnps6) | …[666]GATTGCCTGCTGTCCGCATT[685]… [5184]TACTGGCAAAACCTCCTCTC[5203]… → nps6: 5930 bp, 1960 aa | …[650]CCTATTTAGCGACCCTGATTGCCTGCTGTCCGC–… ---------------CTCCGGCTCCCAG ATGTCTATCA [5223] | 4518 bp deletion 1506 aa deletion
|
SBV4 (Δnps2 Δnps6) | nps2: …[273]AGAACTATACATAGTCTTAT[292]… [8594]TTTGCCGTGGACTGTCACCT[8613]… nps6:…[666]GATTGCCTGCTGTCCGCATT[685]… [5184]TACTGGCAAAACCTCCTCTC[5203]… | nps2: …[260]TGCCTCAGTCTCCAGAACTATACATAGTCT--------------- ----------CCTTGGAAGATACGTC AGACTCA [8633]… nps6: ….[650]CCTATTTAGCGACCCT GATTGCCTGCTGTCCGC--------------------CTCCGGCTCCCAGA TGTCTATCA[5223]…… | 8321 bp deletion 2757 aa deletion 4518 bp deletion 1506 aa deletion |
