Table 1 Characteristics of the three commercially available mRNA transfection reagents provided by the manufacturer.

From: mRNA Transfection-Induced Activation of Primary Human Monocytes and Macrophages: Dependence on Carrier System and Nucleotide Modification

Features provided by the manufacturer

LipoMM

ViroR

ScrF

Material’s chemistry

Lipid-based nanoparticle

Polymeric carrier, based on polycationic PEI core, highly substituted with hydrophobic and anionic side chains

Cationic thioether lipids, containing hydrophobic alkyl groups

Specific structural features

Cationic lipids optimized for mRNA delivery application;

Polymers mimicking viral (influenza hemagglutinin) biophysics

Biomimetic lipid-like molecules made by thiol-yne click chemistry

Tested cell types

Primary cell types such as neurons, fibroblast, hepatocytes and Keratinocytes, specifically tested for mRNA CRISPRs

Primary adherent and suspension cells including monocytes and macrophages and stem cells

Many different human and mouse cell lines such as HEK293, NIH3T3, RAW 264.7 and mouse embryonic stem cells (mESC)

  1. LipoMM: Lipofectamine MessengerMax, ScrF: ScreenFect, ViroR: Viromer RED.
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